By Parviz M. Sabour, Mansel W. Griffiths
Curiosity in bacteriophages as substitute antibacterial brokers in meals grown significantly, according to expanding charges of antibiotic resistance, heightened difficulty with nutrition safeguard, and buyer call for for 'natural' and natural foodstuff items. This new booklet will comprehensively overview the literature at the usage of bacteriophages within the detection and regulate of foodborne bacterial pathogens, either pre-harvest (plant and animal construction) and post-harvest (food processing).
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Extra resources for Bacteriophages in the Control of Food- And Waterborne Pathogens
Y. Børsheim, G. Bratbak, and M. Heldal. 1989. High abundance of viruses found in aquatic environments. Nature 340:467–468. , and H. Shuman. 1998. Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation. Microbiol. Mol. Biol. Rev. 62:204–229. , and I. Herskowitz. 1974. Properties of hybrids between Salmonella phage P22 and coliphage lambda. Nature 251:585–589. Boyd, E. , and H. Bru¨ssow. 2002. Common themes among bacteriophage-encoded virulence factors and diversity among the bacteriophages involved.
A culture of M. 3% sulfuric acid was added to kill extracellular phage. Following neutralization, bacteriophage replication was assayed by addition of a sample to a stationary-phase culture of M. smegmatis mc2155. Following overnight incubation at 37ЊC, MTT was added and the color of each well was recorded after an additional 16 h of incubation. Growth of M. Ⅲ 33 smegmatis in the wells resulted in a color change, whereas for samples where bacterial proliferation was prevented by the lytic action of the phage, no such change was observed.
After accumulation and oligomerization of the holin in the cytoplastic membrane, the holins form a membrane lesion at a genetically predetermined time, permeabilizing the inner membrane for the cognate endolysin. The endolysin, a phage-encoded muralytic enzyme, will degrade the peptidoglycan layer until the cell can no longer withstand the internal os- 18 Ⅲ CEYSSENS AND LAVIGNE motic pressure. , 2000; Wang, 2006). The holin-endolysin two-component cell lysis system described here was long thought to be universal (see chapter 8 in this volume).
Bacteriophages in the Control of Food- And Waterborne Pathogens by Parviz M. Sabour, Mansel W. Griffiths